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詳細(xì)解析！et404毒素試劑盒的檢測原理和步驟
發(fā)布時(shí)間： 2023-02-26 點(diǎn)擊次數(shù)： 1040次et404毒素試劑盒主要是用來檢測毒素的一種經(jīng)濟(jì)、快捷的檢測初篩工具，現(xiàn)在當(dāng)前市面上主要有毒素B1試劑盒、毒素M1試劑盒、毒素總量試劑盒。它的檢測原理：et404毒素試劑盒采用競爭ELISA，在微孔板上預(yù)包被毒素抗原，加入樣本/毒素總量標(biāo)準(zhǔn)品溶液及辣根過氧化物酶標(biāo)記的毒素總量抗體。樣本或標(biāo)準(zhǔn)品溶液中的毒素與預(yù)包被在微孔板上的毒素抗原競爭結(jié)合辣根過氧化物酶標(biāo)記的毒素總量抗體。未結(jié)合的酶標(biāo)抗體在洗滌時(shí)被除去，再加入TMB顯色液，讀取吸光值。樣本的吸光值與其所含殘留物毒素總量的含量成負(fù)相關(guān)。對照標(biāo)準(zhǔn)曲線，即可得出相應(yīng)殘留物毒素總量的含量。et404毒素試劑盒的檢測步驟：樣品處理：取1ml樣品置于5ml離心管中，向離心管中加2ml提取劑，振搖2～3min后，用離心機(jī)以2000r/min，離心5min，取2ml上清液至另一個(gè)5ml離心管中，向其中加2ml凈化劑，振搖5min，用離心機(jī)以2000r/min離心5min，吸取全部下層至點(diǎn)滴板的一個(gè)孔中自然揮干（或加熱將其揮干），備用；樣品檢測：向點(diǎn)滴板上滴加4滴緩沖液，使其充分溶解揮干后的殘留物；取出檢測卡，并做好標(biāo)記，用袋中吸管吸取點(diǎn)滴板上的全部樣液，滴加到檢測卡的加樣孔中（逐滴滴下），5～10min內(nèi)觀察結(jié)果；結(jié)果判斷：陰性：測試線(T)與對照線(C)都出現(xiàn)，表明樣品中毒素的濃度小于0.5μg/kg。陽性：只有對照線(C)，無測試線(T)，表明樣品中毒素的濃度大于或等于0.5μg/kg。無效：對照線(C)和測試線(T)都不出現(xiàn)，或者對照線（C）不出現(xiàn)。
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